ExFHPB-3 Determination of Extraneous Material in Green and Roasted Whole Coffee Beans

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4F4EF73AAE874FDCB3DB9F00A851DEE1

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日期:

2012-3-3

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Published on the Food Directorate’s (Health Canada's) website at,http://www.hc-sc.gc.ca/fn-an/res-rech/analy-meth/microbio/index_e.html.,Government of Canada Gouvernement du Canada,HPB Method ExFHPB-3,February 2006,HEALTH PRODUCTS AND FOOD BRANCH,OTTAWA,DETERMINATION OF EXTRANEOUS MATERIAL IN GREEN AND,ROASTED WHOLE COFFEE BEANS,Evaluation Division, Bureau of Microbial Hazards,Food Directorate, Postal Locator: 2204A1,Health Canada, Ottawa, Ontario K1A 0L2,1. APPLICATION,This method is applicable to the sampling and examination of green and roasted coffee beans for,insect infestation, mould, hairs, fibres, sand, soil and other extraneous material to determine,compliance with Sections 4, 5 and 7 of the Food and Drugs Act. This method replaces ExFHPB-3,dated April 1996.,2. DEFINITION OF TERMS,A lot is defined as that amount (volume, weight, etc.) of the food which is produced, stored and/or,shipped under conditions as nearly uniform as possible, preferably designated by a common container,code or marking, and, in any event, consisting of not more than one variety, grade or type of product,from a single identifiable source.,3. COLLECTION OF SAMPLES,3.1 Scrutinize the lot for live infestation. If live infestation is found, do not sample until after,fumigation or other effective treatment.,3.2 Obtain eight sample units selected at random from the lot of at least 50 g and not more than,100 g each using appropriate sampling equipment and containers. If prepackaged, select,packages that weigh as close to 100 g as possible. Eight sample units constitute a sample.,3.3 Each sample unit must be kept separate and labelled 1, 2, 3, etc. Complete information,respecting the lot size, weight of individual containers, country of origin, exporter, importer,and product and lot identification should be recorded and should accompany the sample.,GREEN COFFEE BEANS,4. MATERIALS AND SPECIAL EQUIPMENT,1) Stereoscopic microscope (10-70x),ExFHPB-3,- 2 - February 2006,2) Compound microscope (100x),5. PROCEDURE,The examination shall be carried out in accordance with the following instructions.,5.1 Analytical Units,5.1.1 Thoroughly mix a sample unit and in a random fashion take 100 beans. These 100 beans,constitute an analytical unit. If a broken bean is found which exceeds one half a bean,consider it as a whole bean. If less than half a bean is encountered, it should be disregarded,and replaced with a whole bean.,5.1.2 Repeat Step 5.1.1 for each of the remaining seven sample units.,5.2 Examination,5.2.1 Examine each coffee bean in the analytical unit with a stereoscopic microscope (10x) for,insect damage, mould, excreta and excrement as outlined in 5.3. Use a higher magnification,only to confirm questionable areas. Confirm mould filaments by transferring questionable,particles to a glass slide and observing with a compound microscope (100x).,5.2.2 Repeat examination for the remaining seven analytical units.,5.3 Classification,5.3.1 Classify each bean into one of the following categories:,(a) Insect Damaged - beans containing insects or their parts, insect tunnels, (circular,holes 0.3-3.0 mm in diameter), excreta, eggs, or webbing. For the purposes of this,method only clearly identifiable insect damage is counted, specifically beans with,bored holes that are characteristic of insect damage. Report the kind and,approximate number of insects and parts if present.,(b) Mouldy - beans containing mould hyphae especially along crevices. Mouldy coffee,beans often have a strong musty odour.,(c) Both Insect Damaged and Mouldy - Do not classify these beans in category (a) or,(b). Mould may be present along insect tunnels.,(d) Excreta and Excrement Contaminated - beans showing bird, rodent or other,mammalian excrement or excreta.,5.3.2 Acceptable Beans: beans that do not show any of the above defects but may show other,quality related damage. Beans showing black, brown or red discolouration should be,classified as defective only if insect and/or mould damage is seen.,5.4 Calculating and Recording Results: ExFHPB-3 GREEN COFFEE BEANS,5.4.1 Record the percent by number of each category of defectives, separately for each analytical,unit, as described in 5.3.1 to the nearest 1%.,ExFHPB-3,- 3 - February 2006,total number of insect,category (a) = % only insect = damaged coffee beans x 100,damaged defectives total number of beans,examined = 100,category (b) = % only mouldy defectives,category (c) = % both insect damaged and mouldy defectives,category (d) = % excreta and/or excrement contaminated,5.4.2 Combine the percent by number of each category to obtain the total percent defectives for,each separate analytical unit:,tot……

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